		Technical Data
F4215-04B	Fibronectin

Description:
Fibronectin seems to play a key role in the tumor cell extracellular matrix during invasive tumor growth. Increasing expression of stromal fibronectin has been reported during tumor progression.

Applications:
Suitable for use in Immunoblot and Immunohistochemistry (frozen tissue and cell preparations, on paraffin-embedded tissue after microwave treatment)

Recommended Dilution:
Immunohistochemistry: 1:5-1:10; Incubation Time 1 hr at room temperature.

Positive Control:
Human skin, ulcerating tissue.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	5F124	Supernatant

Size		Storage	Shipping	Source	Host
1ml		-20°C	Blue Ice	Human	Mouse

Concentration:

Not determined

Immunogen:

Cultivated human fibroblasts.

Purity:

Culture supernatant

Form

Supplied as a liquid.

Specificity:

Antigen recognized human in Species (tested so far). Reacts with the cell binding part of the fibronectin molecule. In immunohistochemistry the antibody is reactive with an extracellular matrix glycoprotein in vessels and connective tissue.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

Christensen L et al: The Distribution of Fibronectin, Laminin and Tetranectin in Human Breast Cancer with Special Attention to Extracellular Matrix. APMIS, suppl. 26, 1-39