Technical Data
F4215-04B
Fibronectin
Description:
Fibronectin seems to play a key role in the tumor cell extracellular matrix during invasive tumor growth. Increasing expression of stromal fibronectin has been reported during tumor progression.

Applications:
Suitable for use in Immunoblot and Immunohistochemistry (frozen tissue and cell preparations, on paraffin-embedded tissue after microwave treatment)

Recommended Dilution:
Immunohistochemistry: 1:5-1:10; Incubation Time 1 hr at room temperature.

Positive Control:
Human skin, ulcerating tissue.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20C or colder. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG15F124Supernatant
SizeStorageShippingSourceHost
1ml-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Cultivated human fibroblasts.
Purity:
Culture supernatant
Form
Supplied as a liquid.
Specificity:
Antigen recognized human in Species (tested so far). Reacts with the cell binding part of the fibronectin molecule. In immunohistochemistry the antibody is reactive with an extracellular matrix glycoprotein in vessels and connective tissue.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Christensen L et al: The Distribution of Fibronectin, Laminin and Tetranectin in Human Breast Cancer with Special Attention to Extracellular Matrix. APMIS, suppl. 26, 1-39