Technical Data
F4215-05A
Fibronectin (FN, Cold-insoluble Globulin, CIG, FN1)
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Technical Data |
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F4215-05A |
Fibronectin (FN, Cold-insoluble Globulin, CIG, FN1) |
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Description: Fibronectins (FNs) constitute a class of high mol. wt. glycoproteins that have a key role to play in various contact processes within vertebrates such as cell attachment and spreading, cell migration, control of cell morphology, differentiation and oncogenic transformation. It is present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. It has a 26aa signal peptide and it undergoes proteolytic processing at its N-terminus to eliminate a 5aa pro-sequence (Ser-Lys-Ser-Lys-Arg). All its biological activities involve its interaction with cells and with extracellular materials like collagen, heparin, fibrin, cell surfaces, bacteria and DNA. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. Plasma FN is secreted by hepatocytes while cellular FN, made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. It maps to 2q34 region of human chromosome. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:50-1:100 Immunohistochemistry (formalin fixed paraffin embedded): 1:25-1:75 Optimal dilutions to be determined by the researcher. Positive Control: Kidney Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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