Technical Data
F4215-05X
Fibronectin (BSA & Azide Free)
Description:
Fibronectin is a dimeric glycoprotein which is present in cells, extracellular matrix, and blood. It possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectin is involved in cell adhesion, tissue organization and wound healing.

Applications:
Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Suitable for use as a Biological Blockade. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1-2ug/ml for 2hrs at RT.
Immunoprecipitation (Native and denatured): 2ug/mg protein lysate; Use Protein G.
Optimal dilutions to be determined by the researcher.

Positive Control:
SW156 cells

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG10.N.295Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human fibronectin purified from serum by affinity chromatography on gelatin-sepharose. MW of Antigen: Non-reduced: 440kD Reduced: 220kD. Epitope: Peptide core. Cellular Localization: Connective tissue matrix and basement membrane.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide, cat# F4215-05.
Specificity:
Recognizes human fibronectin. Reacts with both the plasma and cellular forms of fibronectin. It blocks the fibronectin-mediated cell attachment not by disrupting the collagen-fibrinogen interaction, but by interfering with the attachment of fibronectin to its receptor on the cell surface.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Schoen, R.C., et al., Hybridoma, 1(2): 99-108 (1982). 2. Koch, G.A., et al., Exp. Cell Res., 141: 293-302 (1982).