Technical Data
Fibronectin is a dimeric glycoprotein (~440kD) which is present in cells, extracellular matrix, basement membranes, interstitial connective tissue matrix and blood. It possesses at least four binding sites for collagen, glycosaminoglycans, transglutaminase and a cell surface receptor. Fibronectin is involved in cell adhesion, tissue organization and wound healing. Cellular fibronectin is widely distributed in the stroma of many malignant tumors.

Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot (Reduced and non-reduced): 0.5-1ug/ml for 2hrs at RT.
Immunohistochemistry (Formalin/paraffin): 0.5-1ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG13F179Affinity Purified
500ul4°C (-20°C Glycerol)Blue IceHumanMouse
Recombinant fibronectin protein. Cellular Localization: Connective tissue matrix and basement membrane. MW of Antigen: Non-reduced: 440kD, Reduced: 220kD
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See F4215-10AX.
Recognizes human fibronectin. Species Crosseactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. D’Ardenne AJ, et al. J Clin Pathol 1986; 39:138-44. 2. Kirkpatrick P, et al. J Clin Pathol 1984; 37:639-44. 3. Christensen L. et al. APMIS 1990; 98:615-23. 4. Christiansen BS, et al. Scand J Clin Lab Invest 1988; 48:685-90. 5. S.D.K. Berry et al., J Dairy Science 86:2864-2874