Technical Data
F4215-10A
Fibronectin
Description:
Fibronectin is a dimeric glycoprotein (~440kD) which is present in cells, extracellular matrix, basement membranes, interstitial connective tissue matrix and blood. It possesses at least four binding sites for collagen, glycosaminoglycans, transglutaminase and a cell surface receptor. Fibronectin is involved in cell adhesion, tissue organization and wound healing. Cellular fibronectin is widely distributed in the stroma of many malignant tumors.

Applications:
Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot (Reduced and non-reduced): 0.5-1ug/ml for 2hrs at RT.
Immunohistochemistry (Formalin/paraffin): 0.5-1ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG13F179Affinity Purified
SizeStorageShippingSourceHost
500ul4°C (-20°C Glycerol)Blue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Recombinant fibronectin protein. Cellular Localization: Connective tissue matrix and basement membrane. MW of Antigen: Non-reduced: 440kD, Reduced: 220kD
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide. Also available without BSA and azide. See F4215-10AX.
Specificity:
Recognizes human fibronectin. Species Crosseactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. D’Ardenne AJ, et al. J Clin Pathol 1986; 39:138-44. 2. Kirkpatrick P, et al. J Clin Pathol 1984; 37:639-44. 3. Christensen L. et al. APMIS 1990; 98:615-23. 4. Christiansen BS, et al. Scand J Clin Lab Invest 1988; 48:685-90. 5. S.D.K. Berry et al., J Dairy Science 86:2864-2874