Technical Data
F4215-13A
Fibronectin (FN, Cold-insoluble Globulin, CIG, FN1) (Biotin)
Description:
Plasma Fibronectin is a secreted dimeric protein. It is involved in cell adhesion, migration, wound healing and other cellular processes. Human Fibronectin shares 92% and 90% aa sequence homology with bovine and mouse Fibronectin, respectively.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 0.1-0.2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile TBS, 0.01% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20°CBlue IceHumanSheep
Concentration:
Not determined
Immunogen:
Purified, human plasma-derived, Fibronectin.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 50ug/1ug BSA. Labeled with Biotin. Reconstitute with sterile TBS, pH 7.3, 20mM Trizma, 150mM sodium chloride, 0.1% BSA.
Specificity:
Recognizes human Fibronectin. Species sequence homology: bovine (92%), mouse (90%).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.