Technical Data
F4215-13B
Fibronectin (FN, Cold-insoluble Globulin, CIG, FN1) (APC)
Description:
Fibronectin is an extracellular matrix component that exists in different alternately spliced isoforms. Fibronectin mediates cell adhesion in its insoluble state, but not as a soluble molecule. Fibronectins play a role in cell adhesion, migration, differentiation, and specific gene expression.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 10ul labels up to 1x10e6 cells. For intracellular staining, cells must first be fixed with 4% paraformaldehyde buffer and permeabilized with 0.1% saponin saline buffer.
Optimal dilutions to be determined by the researcher.

Flow Cytometry Protocol:
1. Harvest cells and wash twice in saline buffer.
2. Cell surface staining may be done at this point following the manufacturer’s staining procedure.
3. Resuspend up to 1x10e6 cells in 0.5ml of cold 4% paraformldehyde fixation buffer and incubate at RT for 10 mins.
4. Following fixation, wash the cells twice in saline buffer, then once in 0.1% saponin saline buffer.
5. After permeabilization, add 10ul of F4215-13B and incubate the cells for 30 minutes at RT in the dark.
6. Wash the cells twice with 0.1% saponin saline buffer.
7. Resuspend the cells in saline buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with APC-labeled mouse IgG1 antibody.

Storage and Stability:
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Stable for 12 months at 4°C. Freezing allophycocyanin conjugates will result in a substantial loss of activity. Allophycocyanin conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG111C16Affinity Purified
SizeStorageShippingSourceHost
100Tests4°C Do Not FreezeBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Purified, human plasma Fibronectin.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in saline, 0.5% BSA, 0.1% sodium azide. Labeled with Allophycocyanin (APC).
Specificity:
Recognizes human Fibronectin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.