Technical Data
F4215-44
Fibronectin
Description:
Rabbit polyclonal antibodies against Fibronectin extracted and purified from bovine plasma.

Applications:
Suitable for use in Immunohistochemistry, Immunofluorescence, Western Blot, RIA and ELISA. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry: 1:80 dilution for immunofluorescent staining of frozen bovine skin and liver tissues. Immunohistochemistry on paraffin embedded tissues requires light fixation in 2% PFA, 4% formalin (less than 90 min) acetone or methyl-carnoy fixation, traditional formalin fixation is not recommended. Antigen retrieval is HIER citrate buffer; detection is via enhanced enzymatic methods only.
Western Blot: 1:1000 of 2% deoxycholate and 10% SDS, 6M urea extraced bovine cell cultures. Detects bands at ~220kD.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceBovineRabbit
Concentration:
~1mg/ml
Immunogen:
Fibronectin extracted and purified from bovine plasma.
Purity:
Purified by Protein G chromatography.
Form
Supplied as a liquid in PBS, pH 7.2 No preservatives.
Specificity:
Recognizes bovine Fibronectin. Shows <0.1% reactivity with bovine Collagen Types I and III by radioimmunoassay.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.