Technical Data
Rabbit polyclonal antibodies against Fibronectin extracted and purified from bovine plasma.

Suitable for use in Immunohistochemistry, Immunofluorescence, Western Blot, RIA and ELISA. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry: 1:80 dilution for immunofluorescent staining of frozen bovine skin and liver tissues. Immunohistochemistry on paraffin embedded tissues requires light fixation in 2% PFA, 4% formalin (less than 90 min) acetone or methyl-carnoy fixation, traditional formalin fixation is not recommended. Antigen retrieval is HIER citrate buffer; detection is via enhanced enzymatic methods only.
Western Blot: 1:1000 of 2% deoxycholate and 10% SDS, 6M urea extraced bovine cell cultures. Detects bands at ~220kD.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug4C (-20C Glycerol)Blue IceBovineRabbit
Fibronectin extracted and purified from bovine plasma.
Purified by Protein G chromatography.
Supplied as a liquid in PBS, pH 7.2 No preservatives.
Recognizes bovine Fibronectin. Shows <0.1% reactivity with bovine Collagen Types I and III by radioimmunoassay.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.