		Technical Data
F4215-45	Fibronectin

Description:
Fibronectins are disulfide-linked, dimeric glycoproteins of ~440kD. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase and a cell surface receptor. Fibronectins are involved in cell adhesion, tissue organization and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors.

Applications:
Suitable for use in Immunohistochemistry, ELISA, and RIA. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry: 1:80. IF staining of frozen rat skin and liver tissues.
Optimal dilution to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Purified

Size		Storage	Shipping	Source	Host
100ug		4°C (-20°C Glycerol)	Blue Ice	Rat	Rabbit

Concentration:

~1mg/ml

Immunogen:

Fibronectin extracted and purified from rat plasma.

Purity:

Purified

Form

Supplied as a liquid in PBS, pH 7.2. No preservative added.

Specificity:

Recognizes rat fibronectin. <0.1% reactivity with rat Collagen Types I, II, III, V and rat Elastin by RIA.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.