Technical Data
F4215-47
Fibronectin
Description:
Fibronectin (FN) is involved in many cellular processes, including tissue repair, embryogenesis, blood clotting, and cell migration/adhesion. Fibronectin exists in two main forms: 1) as an insoluble glycoprotein dimer that serves as a linker in the ECM (extracellular matrix), and; 2) as a soluble disulphide linked dimer found in the plasma (plasma FN). The plasma form is synthesized by hepatocytes, and the ECM form is made by fibroblasts, chondrocytes, endothelial cells, macrophages, as well as certain epithelial cells.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.05-1ug/ml. Detected Fibronectin in 15ul of normal rat serum.
Optimal dilution determined by the researcher.

Recommended Secondary Antibodies:
I1904-46E IgG, H&L (HRP) Pab DkxRb
I1904-46K IgG, H&L, X-Adsorbed (HRP) Pab DkxRb

Recommended Positive Control:
Normal rat serum

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceRatRabbit
Concentration:
~0.5mg/ml
Immunogen:
Purified rat plasma fibronectin.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes rat fibronectin at ~262kD. No crossreactivity with other rat plasma proteins as verified via Western Blot analysis. Species Crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Ruoslahti, E., et al., Meth. Enzymol., 82:803 (1982)