		Technical Data
F4215-52	Fibronectin

Description:
Applications:
Immunoblotting at 1:1,000
Indirect immunohistochemistry at 1:200–1:400
A 1-hour fixation with 3–3.5% paraformaldehyde in 0.1 M phosphate buffer pH 7.4 is suggested.
Shows some reactivity on paraffin sections depending on fixation conditions and time.

Storage and Handling: Maintain at 2–8°C for up to 12 months

Type	Isotype	Clone	Grade
Mab	IgG1	2Q606	Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~0.1mg/ml

Immunogen:

Purified fibronectin from Human A8387 fibrosarcoma cells.

Purity:

Purified antibody

Form

Supplied as a liquid in PBS with 1% BSA and 0.1% sodium azide

Specificity:

Specific for the extra domain of cellular fibronectin (EDA sequence) and thus recognizes only the cellular fibronectin. Species Crossreactivity: Human, Rat, rabbit and guinea pig.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Vartio, T., et al., J. Cell Sci. 88:419–430 (1987). 2. Virtanen, I., et al., Histochemistry 90:25–30 (1988). 3. Kuhn, et al., Am. Rev. Respir. Dis. 1693–1703 (1989). 4. Laitinen, L., et al., Lab. Invest. 64:492–498 (1991).