Technical Data
F4215-53R
Fibronectin (FN, Cold-insoluble Globulin) (HRP)
Description:
Fibronectin is a protein which can bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA and actin. Fibronectin is present in the body in two forms; plasma fibronectin (soluble dimeric form) is secreted by hepatocytes, whilst cellular fibronectin (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. Interaction with Tenascin-R mediates inhibition of cell adhesion and neurite outgrowth.

Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:50-1:500
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4░C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20░C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.áLabeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1ml-20░CBlue IceHumanSheep
Concentration:
~1mg/ml
Immunogen:
Fibronectin
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, 0.01% thimerosal. Labeled with Horseradish peroxidase (HRP).
Specificity:
Recognizes human Fibronectin. Species Crossreactivity: canine
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Cho, J. and Mosher, D.F. (2006) Role of fibronectin assembly in platelet thrombus formation. J Thromb Haemost. 4: 1461-9. 2. Wierzbicka-Patynowski, I. and Schwarzbauer, J.E. (2003) The ins and outs of fibronectin matrix assembly. J. Cell Sci. 116 : 3269-76.