Technical Data
F4305-02E
Fibroblast Growth Factor Receptor 1 (FGFR1)
Description:
FGFR1 (Fibroblast Growth Factor Receptor 1) is a member of the fibroblast growth factor receptor family containing an Ig-like domain and a tyrosine kinase domain. This receptor has multiple isoforms and is a Type I membrane protein. FGFR1 is a widely expressed membrane receptor, with distinct isoforms expressed in specific tissues. FGFR1 binds fibroblast growth factor and induces mitogenesis and cellular differentiation. Defects in FGFR1 result in Pfeiffer syndrome associated with craniosynostosis. FGFR1 can be modified by phosphorylation and can bind basic/acidic fibroblast factor depending on the receptor isoform. FGFR1 has been shown to interact with N-cadherin and NCAM.

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1ug/ml recognizes 10ng recombinant bacterial, bacoluviral or native FGFR1.
Immunoprecipitation: Native, nuclear, recombinant and ligand-labeled FGFR1.
Immunohistochemistry: Frozen and paraffin sections.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a8k229Affinity Purified
SizeStorageShippingSourceHost
125ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant human ectodomain of FGFR1a expressed in E. coli beginning with Pro23; antigen contained NH2-terminal Gly-Ser-Pro-Gly-Ile and COOH-terminal Glu-Phe sequences.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4. No preservative added.
Specificity:
Recognizes the alpha isoform of human Fibroblast Growth Factor Receptor 1. Species Crossreactivity: rat and mouse. Epitope: NH2-terminus of unique NH2-terminal Ig loop of FGFR1. Epitope is within the sequence between Glu30 and Ala74 of FGFR1a.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Morrison, R.S., et al., Cancer Res 54: 2794-2799 (1994). 2. Zhao, X.M., et al., J. Clin. Invest. 94: 992-1003 (1994). 3. Feng, S., et al., Biochim. Biophys. Acta 1310: 67-73 (1996). 4. Xu, J., et al., J. Biol. Chem. 267: 17,792-17,803 (1992).