Technical Data
F4305-02G
Fibroblast Growth Factor Receptor 1 (FGFR1, Basic Fibroblast Growth Factor Receptor 1, BFGFR, bFGF-R, CD331, CEK, c-fgr, FGFBR, FGFR-1, FLG, FLJ99988, FLT2, Fms-like Tyrosine Kinase 2, H2, H3, H4, H5, HBGFR, KAL2, N-SAM, OGD)
Description:
FGFR1 (fibroblast growth factor receptor 1) is a member of the fibroblast growth factor receptor family containing an Ig-like domain and a tyrosine kinase domain. This receptor has multiple isoforms and is a Type I membrane protein. FGFR1 is a widely expressed membrane receptor, with distinct isoforms expressed in specific tissues. FGFR1 binds fibroblast growth factor and induces mitogenesis and cellular differentiation. Defects in FGFR1 result in Pfeiffer syndrome associated with craniosynostosis. FGFR1 can be modified by phosphorylation and can bind basic/acidic fibroblast factor depending on the receptor isoform. FGFR1 has been shown to interact with N-cadherin and NCAM.

Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1ug/ml
Flow Cytometry: 1:10-1:1000
Immunohistochemistry: 1:10-1:2000
Immunoprecipitation: 1:10-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG18k231Affinity Purified
SizeStorageShippingSourceHost
125ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant protein corresponding to human ectodomain of FGFR1b, expressed in E. coli, beginning with proline 23; antigen contained NH2-terminal gly-ser-pro-gly-ile and COOH-terminal glu-phe sequences. Cellular Localization: Cell membrane.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4. No preservative added.
Specificity:
Recognizes alpha and beta isoforms of human Fibroblast Growth Factor Receptor 1. Epitope: Within the NH2-terminus of Ig loop II sequence lys145 and glu159 between the acidic box and heparin-binding sequence of FGFR1. Species Crossreactivity: rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Aziz, K.A., et al., Blood 102: 1051-1056 (2003). 2. Feng, S., et al., Biochim. Biophys. Acta 1310: 67-73 (1996). 3. Kan, M., et al., Science 259: 1918-1921 (1993). 4. Xu, J., et al., J. Biol. Chem. 267: 17,792-17,803 (1992).