Technical Data
F4499-50H
Ficolin 2 (Ficolin-B, Ficolin beta, L-ficolin)
Description:
Ficolin-2 is a lectin that contains fibrinogen-like and collagen-like domains. It circulates as a tetramer of disulfide-linked homotrimers. Ficolin-2 associates with MASP proteins to activate complement.

Hybridoma:
Produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human Ficolin-2 (rhFicolin-2).

Applications:
Western Blot, Direct ELISA

Recommended Dilution:
Western Blot: This antibody can be used at 1-2ug/mL with the appropriate secondary reagents to detect human Ficolin-2. Using a colorimetric detection system, the detection limit for rhFicolin-2 is approximately 25 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommended. Chemiluminescent detection with Chemiluminescent Detection Substrate will increase sensitivity by 5 to 50 fold.
Direct ELISA: This antibody can be used at 0.5-1.0ug/mL with the appropriate secondary reagents to detect human Ficolin-2. The detection limit for rhFicolin-2 is approximately 1 ng/well.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b8H139Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
NS0-derived rhFicolin-2
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitution: Reconstitute with sterile PBS. If 0.2ml of PBS is used, the antibody concentration will be 500ug/ml.
Specificity:
Recognizes human Ficolin-2. Does not crossreact with rhFicolin-3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.