		Technical Data
F4568-01A	Flotillin 1 (FLOT1, Reggie 2)

Description:
Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined.

Cellular Localization: Cell Membrane

Positive Control: H460 lysate

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide: SISQVNHKPLRTA, representing the C-terminus of the human protein (residues 415-427), according to NP_005794.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in TBS, pH 7.2, 0.5% BSA.

Specificity:

Species Crossreactivity: Crossreacts with Human. Expected to crossreact with Chimpanzee and porcine due to sequence homology.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

Bickel PE et al. Flotillin and epidermal surface antigen define a new family of caveolae-associated integral membrane proteins. J Biol Chem 272:13793-802 (1997).