Technical Data
F4568-01B
Flotillin 1 (FLOT1, Reggie 2)
Description:
Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined.

Applications:
Suitable for use in ELISA and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Peptide ELISA Titer: 1:32,000
Western Blot: 0.3-1ug/ml ~48kD band observed in lysates of cell line H460 (calculated MW of 47.4kD according to NP_005794.1). In transfected HEK293 transiently expressing FLOT1 a band of approx. 48kD is observed. This band is not observed in the non-transfected HEK293.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-SISQVNHKPLRTA, from the C Terminus of human Flotillin 1 (NP_005794.1).
Purity:
Purified by peptide affinity chromatography.
Form
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes human Flotillin 1. Species sequence homology: canine, porcine, bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Vassilieva EV, Gerner-Smidt K, Ivanov AI, Nusrat A.Lipid rafts mediate internalization of beta1-integrin in migrating intestinal epithelial cells.Am J Physiol Gastrointest Liver Physiol. 2008 Nov;295(5):G965-76.
PMID: 187558112.The goat polyclonal antibody used in this paper was manufactured by us: Masilamani M, Narayanan S, Prieto M, Borrego F, Coligan JE.Uncommon endocytic and trafficking pathway of the natural killer cell CD94/NKG2A inhibitory receptor.Traffic. 2008 Jun;9(6):1019-34.PMID: 18363778 3. Dolganiuc A, Bakis G, Kodys K, Mandrekar P, Szabo G.Acute ethanol treatment modulates Toll-like receptor-4 association with lipid rafts. Alcohol Clin Exp Res. 2006 Jan;30(1):76-85.
Alcohol Clin Exp Res. 2006 Jan;30(1):76-85.