Technical Data
Media
Fluid Thioglycollate Medium w/K Agar (Powder)
F5025
Microbiological Grade
Storage RT/4C    Shipping RT
Components are shown as g/Liter:
L-Cystine0.5
Dextrose5.0
K Agar0.45
Potassium Chloride2.5
Resazurin0.001
Sodium Chloride0.5
Sodium Thioglycollate 0.5
Casitone15.0
Yeast Extract5.0
Total: 29.451g/L
500g makes 16.98L
A sterility testing medium where clarity of the medium is required, Fluid Thioglycollate Medium w/K Agar is suitable for isolation of A. acidoterrestris spores from fruit juices. K Agar is a native form that exhibits exceptional clarity in gel form, allowing for easier visual detection of bacterial growth. Dextrose, casitone, cystine, and yeast extract provide the growth factors necessary for bacterial replication. Sodium provides essential ions. The reducing agents sodium thioglycollate and cystine lower the redox potential of the medium, thereby supporting anaerobiosis. Sodium thioglycollate also prevents accumulation of peroxides which are lethal to some microorganisms. Resazurin serves as redox indicator.


Directions per Liter: Dissolve 29.45g in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Note: After storage of the medium, if 30% of the medium demonstrates oxidation (color change of indicator), reheat the medium once in boiling water or steam bath. Storage and Stability:Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Dehydrated Appearance: Light tan, homogeneous free flowing powder

Prepared Appearance: Amber/Yellow (top 10% light pink to medium red), clear

pH: As Reported


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.