Technical Data
F5250-09
Fluorescein Isothiocyanate (FITC)
Description:
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.

Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:10 -1:2,000 (frozen and paraffin)
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a8k242Affinity Purified
SizeStorageShippingSourceHost
500ug4C Do Not FreezeBlue IceMouse
Concentration:
~4mg/ml
Immunogen:
FITC-BSA conjugate.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS. pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes both free fluorescein (FITC) and conjugated FITC, so it recognizes FITC-labeled proteins. Kd = 1.5 x 10e8.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.