		Technical Data
F5250-10	Fluorescein Isothiocyanate (FITC)

Description:
Fluorescein isothiocyanate (FITC) is a small uorescent molecule that is commonly conjugated to antibodies for immuno uorescent cell labeling. In conjunction with anti-FITC antibodies, it can also be used as an epitope tag for detection and signal ampli cation.

Applications:
Suitable for use in Immunofluorescent staining, ELISA, Western Blot, Dot and Slot Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
1mg		-20°C	Blue Ice		Sheep

Concentration:

~1mg/ml

Immunogen:

Fluorescein isothiocyanate (Isomer I)

Purity:

Purified by affinity chromatography.

Form

Supplied as a liquid in 100mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added.

Specificity:

Recognizes fluorescein isothiocyanate. Cross-absorbed against human, mouse and rat serum proteins.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.