Technical Data
F5735
Fodrin, alpha (Spectrin Non-erythroid alpha Chain, Alpha II Spectrin, FLJ44613, Spectrin alpha Non-erythrocytic 1, SPTAN1, SPTA2)
Description:
In non-erythroid cells, a spectrin-like protein has been identified as fodrin and found to contain two subunits (alpha subunit of M.W. 240kD and beta subunit of M.W. 235kD). Fodrin is believed to be involved in 1) stabilizing membrane structure; 2) maintaining cell shape; 3) linking the cytoskeleton to plasma membranes or intracellular vesicles. In particular, fodrin is known to mediate the association of actin filaments with the plasma membrane.

Appplications:
Suitable for use in Western Blot, ELISA, Immunoprecipitation, Immunocytochemistry, Flow Cytometry, Immunohistochemistry and RIA. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:200
ELISA: 1:1000
Immunoprecipitation: 1:100
Immunocytochemistry: 1:100
Flow Cytometry: 1:500
Immunohistochemistry: 1:100
RIA: 1:5000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a2Q236Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceBovineMouse
Concentration:
~0.5mg/ml
Immunogen:
Purified bovine brain fodrin
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.5, 0.01% sodium azide.
Specificity:
Recognizes the bovine intracellular fodrin (240kD). Species Crossreactivity: human, mouse, rabbit, rat, canine and porcine
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Int. Rev. Cytol. 87: 195-224 (1984). 2. J. Cell Biol. 101: 477-487 (1985). 3. Physiological Reviews 70: 1029-1054 (1990). 4. Cell Motil. and the Cytoskeleton 12: 225-247 (1989). 5. J. Biol. Chem. 267: 22,073-22,078 (1992).