Technical Data
F5753
Folate Receptor alpha (FRa)
Description:
The folate receptor is a highly selective tumor marker overexpressed in greater than 90% of ovarian carcinomas.

Applications:
Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1-10ug/ml
ELISA: 1-10ug/ml
Western Blot: 1-10ug/ml. Avoid reducing reagents; use 0.1% SDS in transfer buffer.
Immunoprecipitation: 1-10ug/ml
Immunohistochemistry (Frozen): 1-10ug/ml. Not suitable for paraffin embedded tissues.
Immunocytochemistry: 1-10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG16D398Highly Purified
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Membrane preparation from a human ovarian carcinoma specimen.
Purity:
Purified ~95% (SDS-PAGE)
Form
Supplied as a liquid in PBS, pH 7.4. No preservatives added.
Specificity:
Recognizes membrane-bound and soluble human folate receptor (FR) alpha. Does not crossreact with mouse or rat folate receptor. Does not crossreact with the beta-isoform. Species Crossreactivity: monkey.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Basal, E., et al., PLoS ONE 4: e6292 (2009). General References: 1. Miotti, S., et al., Int. J. Cancer 39: 297 (1987). 2. Coney, L.R., et al., Cancer Res. 5: 6125 (1991). 3. Weitman, S.D., et al., Cancer Res. 52: 3396 (1992). 4. Gesmundo, N., et al., Eur. J. Biochem. 217: 337 (1993). 5. Tomassetti, A., et al., FEBS Lett. 317: 143 (1993). 6. Mantovani, L.T., et al., Eur. J. Cancer 30A: 363 (1994). 7. Corona, G., et al., Int. J. Cancer 75: 125 (1998). 8. Tomassetti, A., et al., J. Cell Biochem. 72: 111 (1999).