Technical Data
F5753
Folate Receptor alpha (FRa)
Description:
The folate receptor is a highly selective tumor marker overexpressed in greater than 90% of ovarian carcinomas.

Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1-10ug/ml
Western Blot: 1-10ug/ml. Avoid reducing reagents; use 0.1% SDS in transfer buffer.
Immunoprecipitation: 1-10ug/ml
Immunohistochemistry (Frozen): 1-10ug/ml. Not suitable for paraffin embedded tissues.
Immunocytochemistry: 1-10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG16D398Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Membrane preparation from a human ovarian carcinoma specimen.
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.4. No preservatives added.
Specificity:
Recognizes membrane-bound and soluble human folate receptor (FR) alpha. Does not crossreact with mouse or rat folate receptor. Does not crossreact with the beta-isoform. Species Crossreactivity: monkey.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Basal, E., et al., PLoS ONE 4: e6292 (2009). General References: 1. Miotti, S., et al., Int. J. Cancer 39: 297 (1987). 2. Coney, L.R., et al., Cancer Res. 5: 6125 (1991). 3. Weitman, S.D., et al., Cancer Res. 52: 3396 (1992). 4. Gesmundo, N., et al., Eur. J. Biochem. 217: 337 (1993). 5. Tomassetti, A., et al., FEBS Lett. 317: 143 (1993). 6. Mantovani, L.T., et al., Eur. J. Cancer 30A: 363 (1994). 7. Corona, G., et al., Int. J. Cancer 75: 125 (1998). 8. Tomassetti, A., et al., J. Cell Biochem. 72: 111 (1999).