Technical Data
F5800-11
Folic Acid (Vitamin B9, Pteroylglutamic acid), Conjugated
Description:
Applications:
Suitable for use in Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry: 1:500-1:1000.
Optimal dilutions to be determined by the researcher.

Specificity:
Using a conjugate Folic acid-protein carrier (BSA), antibody specificity was performed with an ELISA test by competition experiments with the following compounds :

Compound Cross-reactivity ratio (a)
Folic acid-BSA 1
Folinic acid-BSA 1:>900
Tetrahydrofolic acid-BSA 1:>2,000
Methotrexate-BSA 1:>50,000
Riboflavin-BSA 1:>50,000

(a): Folic acid-BSA concentration/unconjugated or conjugated compound concentration at half displacement.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
100ug-20CBlue IceRat
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
Synthetic Folic Acid conjugated to BSA.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder in PBS, pH 7.2. No preservative added. Reconstitute with 100ul sterile dH2O.
Specificity:
Recognizes protein-conjugated Folic Acid (Vitamin B9). See additional crossreactivity data.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.