Technical Data
Folic Acid (Vitamin B9, Pteroylglutamic acid), Conjugated
Suitable for use in ELISA, Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
ELISA: 1:2000-5000
Immunohistochemistry: 1:500-1000. Folic Acid-immunoreactive fibers located in the ventro-lateral part of the pulvinar nucleus of monkey brain. Also, immunoreactive fibers containing Folic acid in the dorsal mesencephalic region of the monkey brain. Fibers located between the ventricul and the lateral geniculate nucleus of the thalamus.
Optimal dilutions to be determined by the researcher.

Using a conjugate Folic acid-protein carrier (BSA), antibody specificity was performed with an ELISA test by competition experiments with the following compounds :

Compound Cross-reactivity ratio (a)

Folic acid-BSA 1
Folinic acid-BSA 1/>900
Tetrahydrofolic acid-BSA 1/>2,000
Methotrexate-BSA 1/>50,000
Riboflavin-BSA 1/>50,000
BSA 1/>50,000

(a): Folic acid-BSA concentration/unconjugated or conjugated compound concentration at half displacement.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ug-20CBlue IceRat
As reported
Synthetic Folic Acid conjugated to BSA.
Purified by immunoaffinity chromatography.
Supplied as a lyophilized powder in PBS, pH 7.2. No preservative added.
Recognizes protein-conjugated Folic Acid (Vitamin B9). See additional crossreactivity data.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.