Technical Data
F6072-10C
Fragile X Mental Retardation Protein 1 (FMR1, FMR-1, FMRP, FRAXA, MGC87458, POF, POF1, Protein FMR-1)
Description:
Fragile X Mental Retardation Protein (FMRP) is a RNA-binding protein that is associated to polysomes and may be involved in the transport of mRNA from the nucleus to the cytoplasm. Defects in FMR1 are the cause of Fragile X syndrome, which is a common genetic disease characterized by moderate to severe mental retardation, macroorchidism, large ears, prominent jaw, and high-pitched, jocular speech. The defect in most fragile X syndrome patients results from an amplification of a CGG repeat region which is directly in front of the coding region.

RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation.

Applications:
Suitable for use in ELISA, Western Blot, Immunocytochemistry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilution:l
ELISA: 1:500-5000
Western Blot: 1:500
Immunocytochemistry: 1:500-5000
Immunohistochemistry (Frozen and paraffin): 1:500-5000
Optimal dilutions to be determined by the researcher.

Positive Control:
Includes negative control mouse IgG antibody and control primers specific for mouse Iqsec1.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k10H36Ascites
SizeStorageShippingSourceHost
10Tests4C/-20CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
RFusion protein with a full length FMRP (human).
Purity:
Ascites
Form
Supplied as a liquid.
Specificity:
Recognizes human FMRP. Species Crossreactivity: mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.