Technical Data
F6401-01A
Frizzled 1 (FZD1)
Description:
Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Direct ELISA: 0.5-1ug/ml. Detection limit is ~0.6ng/well.
Western Blot: 0.1-0.2ug/ml. Detection limit for rmFrizzled-1 is ~5ng/lane under non-reducing and reducing conditions.
Immunohistochemistry: 2-15ug/ml. Detects Frizzled-1 in cells and tissues.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseGoat
Concentration:
~0.1mg/ml
Immunogen:
NS0-derived, recombinant mouse Frizzled-1, extracellular, Cysteine-Rich Domain (CRD).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 5% trehalose.
Specificity:
Recognizes mouse Frizzled-1 extracellular Cysteine-Rich Domain (CRD). Crossreactivity: rmFrizzled-2, rmFrizzled-4, rmFrizzled-7 and rmFrizzled-8: < 10%; rmFrizzled-3 and rmFrizzled-6: < 1%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.