Technical Data
Frizzled 1 (FZD1)
Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway and are involved in a variety of developmental processes. Frizzled proteins each contain a divergent N-terminal signal peptide, a highly conserved extracellular cysteine-rich domain, a variable-length linker region, a seven-pass transmembrane domain and a variable-length C-terminal tail.

Fusion of a mouse myeloma with B cells obtained from a rat immunized with purified, NS0-derived, recombinant mouse Frizzled-1 (rmFrizzled-1) extracellular domain.

Suitable for use in Western Blot and Direct ELISA. Other applications not tested.

Recommended Dilution:
Western Blot: Use at 1-2ug/ml with the appropriate secondary reagents to detect mouse Frizzled-1. Using a colorimetric detection system, the detection limit for rmFrizzled-1 is ~5ng/lane and 1ng/lane under non-reducing and reducing conditions, respectively.
Direct ELISA: Used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect mouse Frizzled-1. The detection limit for rmFrizzled-1 is ~5ng/well.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG15A2Affinity Purified
500ug-20CBlue IceMouseRat
NS0-derived rmFrizzled-1 extracellular domain
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, pH 7.2, with 5% trehalose.
Recognizes mouse Frizzled-1. Shows no crossreactivity with rmFrizzled-2, -3, -4, -6, -7 or -8.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.