Technical Data
Frizzled 8 (FZD8)
FZD8 is a Frizzled Receptor that mediates Wnt signaling and is involved in determining vertebrate axis and tissue polarity. FZD8 is expressed during embryonic development in forming and differentiating somites in mice. FZD8 expression has been reported in brain, heart, kidney, pancreas, and skeletal muscle. ESTs have been isolated from embryo, eye, head/neck, heart, kidney, liver/spleen, pancreas, prostate, and testis libraries, as well as many tumor libraries.

Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Direct ELISA: 0.5-1ug/ml. The detection limit is ~2ng/well.
Western Blot: 0.1-0.2ug/ml. The detection limit is ~5ng/lane under non-reducing and reducing conditions.
Immunohistochemistry: 5-15ug/ml detects FZD8 in cells and tissues.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceMouseGoat
NS0-derived recombinant mouse Frizzled-8 extracellular Cysteine-Rich Domain (CRD)
Purified by affinity chromatography.
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with1ml sterile PBS.
Recognizes recombinant mouse Frizzled-8. Species Crossreactivity: recombinant mouse Frizzled-9: ~5%; recombinant mouse Frizzled-1,-2, -3, -4, -6 and -7: <1%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.