Technical Data
F9050-10K
FOXP3 (Forkhead Box P3, AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID)
Description:
FOXP3 is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis.
FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Frozen): 1:50. Tested on mouse thymus from an influenza-infected mouse or thymus from healthy mouse.
Western Blot: 1:1000-1:5000 detects a band at~47.2kD
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
~0.1mg/ml
Immunogen:
A synthetic peptide corresponding to a region between aa400-431 at the C-terminal region of human FOXP3 (Q9BZS1)
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris-citrate/phosphate, pH 7.5, 0.1% sodium azide.
Specificity:
Recognizes human FOXP3. Species Crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Polanczyk MJ, et al. Cutting edge: estrogen drives expansion of the CD4+CD25+ regulatory T cell compartment. J Immunol. 173(4:2227-30 (2004) 2. Ochs, HD, et al. FOXP3 acts as a rheostat of the immune response. Immunol Rev. 203:156-64 (2005)