Technical Data
F9050-10K
FOXP3 (Forkhead Box P3, AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID)
Description:
FOXP3 is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis.
FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.

Applications:
Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000-1:5000 detects a band at ~47.2kD
Immunofluorescence (IC): 1:10-1:500
Immunohistochemistry: 1:50-1:1000. Tested on mouse thymus from an influenza-infected mouse or thymus from healthy mouse.
Immunohistochemistry (Paraffin): 1:200-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul4C Do not freezeBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
A synthetic peptide corresponding to a region between aa400-431 at the C-terminal region of human FOXP3 (Q9BZS1)
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris-citrate/phosphate, pH 7.5, 0.09% sodium azide.
Specificity:
Recognizes human FOXP3. Species Crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Polanczyk MJ, et al. Cutting edge: estrogen drives expansion of the CD4+CD25+ regulatory T cell compartment. J Immunol. 173(4):2227-30 (2004) 2. Ochs, HD, et al. FOXP3 acts as a rheostat of the immune response. Immunol Rev. 203:156-64 (2005) 3. Ochando, JC et al. J Immunol;174(11):6993-7005. 2005 Jun 1. [PMID: 15905542]. 4. Rahman S, Gudetta B, Fink J et al. Am J Pathol;174(6):2211-24. 2009 Jun.