Technical Data
F9050-12
FOXP3 (Forkhead Box P3, AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID)
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Technical Data |
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F9050-12 |
FOXP3 (Forkhead Box P3, AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID) |
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Description: FoxP3 is a 48kD forkhead transcription factor family member, which was originally identified as the novel protein scurfin. FoxP3 functions as a DNA binding protein that represses transcription, and is reported to be involved in the regulation of T cell activation, differentiation and homeostasis. FoxP3 is predominantly expressed within the nuclei of CD25 +CD4 + regulatory T cells (Tr). This population of FoxP3+CD25+CD4+ Tr cells plays an essential role in controlling autoimmunity. Applications: Suitable for use in ELISA, Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications have not been tested. Recommended Dilutions: ELISA (Direct and indirect): 1:2000-1:10,000 Western Blot: 1:2000-1:5000 using ECL. Suggested blocking and dilution buffer is TBST, 0.05% Tween-20 and 5% skim milk. Suggested incubation time is 1 hour at RT. Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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