Technical Data
F9050-12
FOXP3 (Forkhead Box P3, AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID)
Description:
FoxP3 is a 48kD forkhead transcription factor family member, which was originally identified as the novel protein scurfin. FoxP3 functions as a DNA binding protein that represses transcription, and is reported to be involved in the regulation of T cell activation, differentiation and homeostasis. FoxP3 is predominantly expressed within the nuclei of CD25 +CD4 + regulatory T cells (Tr). This population of FoxP3+CD25+CD4+ Tr cells plays an essential role in controlling autoimmunity.

Applications:
Suitable for use in ELISA, Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications have not been tested.

Recommended Dilutions:
ELISA (Direct and indirect): 1:2000-1:10,000
Western Blot: 1:2000-1:5000 using ECL. Suggested blocking and dilution buffer is TBST, 0.05% Tween-20 and 5% skim milk. Suggested incubation time is 1 hour at RT.
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a6D401Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceMouseRat
Concentration:
~0.5mg/ml
Immunogen:
Recombinant protein corresponding to mouse FOXP3 (forkhead box protein P3).
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.4. No preservative added.
Specificity:
Recognizes both natural and recombinant mouse FOXP3. Detects a band of ~46-49kD by Western Blot.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ambrosino, et al.; Cancer Res. 66, 7734 (2006) 2. G. Raimondi, et al.; J. Immunol. 176, 2808 (2006). 3. M.J. Perone, et al.; J. Immunol. 177, 5278 (2006). 4. Y.Y. Lan, et al.; J. Immunol. 177, 5868 (2006).