Technical Data
Media
Fungisel Agar (Powder)
F9550
Microbiological Grade
Storage 4C    Shipping RT
Components are shown as g/Liter:
Papaic Digest of Soybeans10.0
Dextrose10.0
Chloramphenicol0.05
Cycloheximide0.4
Agar15.5
Total:36g/Liter
Selective medium with nearly neutral pH, used to isolate pathogenic fungi.

Description:
Fungisel agar contains chloramphenicol and cycloheximide. These antibiotics selectively inhibit saprophytic fungi and bacteria while allowing pathogenic fungi to grow. Various workers demonstrated the use of these antibiotics in Sabouraud Dextrose Agar and other media for the isolation of pathogenic fungi from specimens with mixed flora.

Storage:
Store the sealed bottle containing the dehydrated medium at 2 to 8.0C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.


Directions: 1. Dissolve 36grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized.

2. Distribute and autoclave for at 121.0C for 15 minutes. Cool to room temperature, use immediately. Do not remelt more than once. Do not hold medium at 45-50C for more than 30 minutes. DO NOT OVERHEAT. Storage:Store powdered media at 4C. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be stored at 4C and used within a short period of time.

Appearance: Light beige, homogeneous, free flowing powder.

Solubility: Light to medium yellow, hazy to clear, complete.

pH (25C): 6.90.2

Microbiological Analysis: Aspergillus niger ATCC 16404


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.