Technical Data
G1015-05A
GABA (Gamma-aminobutyric Acid), Conjugated
Description:
GABA is a major inhibitory neurotransmitter, the GABAergic transmission being terminated by the rapid Na+/Cl-dependent uptake through GABA transporters. It has been subdivided into neural and glial uptake systems on the basis of pharmacological properties. Recently, molecular cloning studies have identified multiple subtypes of GABA transporters (GAT1, GAT2, GAT3; and betaine GABA transporter (BGT-1). There is ~50% homology between various GABA transporter subtypes. GABA transporters are predicted to contain 12 potential transmembrane domains. The NH2 and COOH-termini are predicted to be intracellular. Two of the high affinity (Km~10 uM) rat GABA transporters (GAT2 and GAT3/GAT-B) share higher amino acid identity (68% and 65%, respectively) with the kidney betaine transporter than with GAT-1 (52% aa identity). GAT1 and GAT3 have been detected in various parts of the brain while GAT2 is found in many tissues. It appears that GAT1 and GAT3 are involved in distinct GABAergic transmission while GAT2 may be important in non-neural functions.

Applications:
Suitable for use in ELISA, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:2000-1:5000. Tested using the free floating PAP technique on rat hippocampus and cerebellum. The anti-conjugated GABA antibodies stains.

Specificity
Using a conjugate GABA-Glutaraldehyde-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds :

Compound Cross-reactivity ratio (a)
GABA-G-BSA 1
b-Alanine-G-BSA 1/10,000
Glycine-G-BSA 1/20,000
D-Aspartate-G-BSA 1/> 100,000
D-Glutamate-G-PPL 1/> 10,000

(a) : GABA-G-BSA concentration/unconjugated or conjugated aminoacid concentration at half displacement;
G = Glutaraldehyde

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
100ug-20°CBlue IceRabbit
Concentration:
~1mg/ml
Immunogen:
GABA-Glutaraldehyde-BSA
Purity:
Antiserum previously preabsobed on protein carriers, and purified by ammonium sulfate precipitation and gel filtration.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added.
Specificity:
Recognizes conjugated GABA. See additional crossreactivity data.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

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o LESCAUDRON L., SEGUELA P., GEFFARD M. and VERNA A. Effects of long term ethanol consumption on gabaergic neurons in the mouse hippocampus : a quantitative immunocytochemical study. Drug and alcohol dependence, (1986) 18, 377-384.
o RABHI M., ONTENIENTE B., KAH O., GEFFARD M. and CALAS A. Immunocytochemical study of the GABAergic innervation of the mouse pituitary by use of antibodies against gamma-aminobutyric acid (GABA). Cell & Tissue Res., (1987) 247, 33-40.
o BUIJS R.M., VAN VULPEN E.H.S. and GEFFARD M. Ultrastructural localization of GABA in the supraoptic nucleus and neural lobe. Neuroscience, (1987) 20, 347-355.
o MAGOUL R., ONTENIENTE B., GEFFARD M. and CALAS A. Anatomical distribution and ultrastructural organization of the Gabaergic system in the rat spinal cord. An immunocytochemical study using anti-GABA antibodies. Neurosc., (1987) 20, 1001-1009.
o ONTENIENTE B., GEFFARD M., CAMPISTRON G. and CALAS A. An ultrastructural study of GABA-immunoreactive neurons and terminals in the septum of the rat. J. Neurosc., (1987) 7, 48-54.
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o ESCLAPEZ M., CAMPISTRON G., and TROTTIER S. Immunocytochemical localization and morphology of GABA-containing neurons in the prefrontal and frontoparietal cortex of the rat. Neurosc. Letters, (1987), 77, 131-136.
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o SCHIFFMANN S., CAMPISTRON G., TUGENDHAFT P., BROTCHI J., FLAMENT-DURAND J., GEFFARD M. and VANDERHAEGHEN. Immunocytochemical detection of GABAergic nerve cells in the human temporal cortex using a direct g-aminobutyric acid antiserum. Brain Res., (1988), 442, 270-278.
o GILON P., CAMPISTRON G., GEFFARD M. and REMACLE C. Immunocytochemical localization of GABA in endocrine cells of the rat entero-pancreatic system. Biology of the Cell. (1988), 62, 265-273.
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o DECAVEL C., DUBOURG P., LEON-HENRI B., GEFFARD M. and CALAS A. Simultaneous immunogold labeling of GABAergic terminals and vasopressin-containing neurons in the rat paraventricular nucleus. Cell & Tissue Res., (1989), 255, 77-80.
o KITAHAMA K., SALLANON M., OKAMURA H., GEFFARD M. et JOUVET M. Cellules présentant une immunoréactivité au GABA dans l'hypothalamus du chat. C.R. Acad. Sci., (1989), 308, 507-511.
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o TROTTIER S., GEFFARD M. and EVRARD B. Co-localization of tyrosine hydroxylase and GABA immunoreactivities in human cortical neurons. Neurosc. Letters (1989), 106, 76-82.
o PITUELLO F., KAN P., GEFFARD M., and DUPRAT A. Initial GABAergic expression in embryonic amphibian neuroblasts after neural induction. Int. J. Dev. Biol., (1989) 33, 445-453.
o MARTINOLI M.G., DUBOURG P., GEFFARD M., CALAS A. and KAH O. Distribution of GABA-immunoreactive neurons in the forebrain of the goldfish, Carassius auratus. Cell and Tissue Res., (1990) 260, 77-84.
o GILON P., MALLEFET J., DE VRIEND C., PAUWELS S., GEFFARD M., CAMPISTRON G and REMACLE C. Immunocytochemical and autoradiographic studies of the endocrine cells interacting with GABA in the rat stomach. Histochemistry, (1990) 93, 645-654.
o DUPONT J., GEFFARD M., CALAS A. and ARAN J.M. Immunohistochemical evidence for GABAergic cell bodies in the medial nucleus of the trapezoid body and in the lateral vestibular nucleus in the guinea pig brainstem. Neuroscience Letters, (1990) 111, 263-268.
o PHILIPPE E., GAULIN F., DELAGRAVE C. and GEFFARD M. Expression of GABA-immunoreactivity by spinal motoneurons of some vertebrates, Neurosci. Letters, (1990) 116, 12-16.
o VERNEY C., ALVAREZ C., GEFFARD M. and BERGER B. Ultrastructural double-labelling study of dopamine terminals and GABA-containing neurons in rat anteromedial cerebral cortex. Europ. J. of Neurosc., (1990) 2, 960-972.
o BENNIS M., CALAS A., GEFFARD M. and GAMRANI H. Distribution of GABA immunoreactive systems in the forebrain and midbrain of the chameleon. Brain Res. Bull., (1991) 26, 891-898.
o DUBREIL V., SINAKEVITCH I.G., HUE B. and GEFFARD M. Neuritic GABAergic synapses in insect neurosecretory cells. Neurosc. Res., (1994) 19, 235-240.
o LAALAOUI A., CHOUAF L., DIDIER-BAZES M., GEFFARD M., BELIN M.F. and GAMRANI H. GABA uptake and phenotypic characteristics of the subcommissural ependymocytes of the semi-desertic rodent, Meriones shawi : correlation with serotoninergic innervation. Cell Tissue Res. (1996) 285(3), 435-443.