Technical Data
GABA (Gamma-aminobutyrIc Acid)
Gamma-aminobutyric acid is the chief inhibitory neurotransmitter in the mammalian central nervous system. It plays a role in regulating neuronal excitability throughout the nervous system. In humans, GABA is also directly responsible for the regulation of muscle tone. Although chemically it is an amino acid, GABA is rarely referred to as such in the scientific or medical communities, because the term "amino acid," used without a qualifier, conventionally refers to the alpha amino acids, which GABA is not, nor is it ever incorporated into a protein. In spastic diplegia in humans, GABA absorption becomes impaired by nerves damaged from the condition's upper motor neuron lesion, which leads to hypertonia of the muscles signaled by those nerves that can no longer absorb GABA.

Suitable for use in ELISA and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry (FFPE): 1:500 using heat-induced epitope retrieval
Optimal dilutions to be determined by the researcher.

Recommended Positive Control:
Rat brain tissue

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
50ul-20°CBlue IceGuinea pig
Not Determined
GABA coupled to BSA via glutaraldehyde
Supplied as a liquid in 0.1M Tris-Glycine, 0.15M sodium chloride, pH 7.4 0.05% sodium azide
Recognizes GABA. Staining was blocked by preabsorbing with 100uM GABA conjugated to glutaraldehyde. 500uM of similar conjugations of glutamic acid, glutamate and taurine failed to block staining. Species Crossreactivity: rat. Expected to react with all species.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. McDonald, A., Neurosci. Lett. 100: 53 (1989). 2. Roettger, V. and Goldfinder, M., Neurosci. Lett. 97: 46 (1989). 3. Johnson, M. and Vardi, N., Visual Neuroscience 15: 743-753 (1998). 4. Hantman, A.W., et al., J. Neuroscience 24: 836-842 (2004). 5. Xu, M., et al., J. Neuroscience 24: 4576-4584 (2004). 6. Watanabe, M., et al., Int. Rev. Cytol. 213: 1-47 (2002).