Technical Data
G1015-06
GABA Receptor A, alpha 1 (Gamma-aminobutyric Acid Receptor Type A alpha 1 Subunit, GABRA1)
Description:
The neurotransmitter GABA binds to specific transmembrane receptors in the plasma membrane of both pre- and postsynaptic neurons. The GABAA receptor is a multiprotein complex that acts as a ligand-gated chloride channel. There are at least 6 different isoforms of the alpha subunit. Increased alpha1 subunit assembly in postsynaptic receptors of cerebellar inhibitory synapses is responsible for the fast inhibitory synaptic currents observed during postnatal development. Additionally, alpha1-containing GABAA receptors are important in regulating the reinforcing properties of alcohol, and expression of the alpha1 subunit increases upon chronic exposure to alcohol, making it a potential therapeutic target for alcohol dependency.

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:10,000 detects the GABA Receptor A, alpha1 subunit in 10ug of rat brain microsomal preparation. tissue lysate. Proteins were visualized using an IgG (HRP) donkey anti-rabbit and a chemiluminescence detection system.
Immunoprecipitation: 5ul immunoprecipitates the GABA Receptor A, alpha1 subunit in rat brain microsomal preparation RIPA lysate.
Immunohistochemistry (Paraffin): 1:500 detects the GABA Receptor A, alpha1 subunit in rat hippocampus tissue. Requires HIER.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb
I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceRatRabbit
Concentration:
~0.5mg/ml
Immunogen:
Linear peptide corresponding to the topological domain of rat GABA Recptor A, alpha1, conjugated to KLH. (NP_899155). Species Sequence Homology: human, 94%
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes rat GABA Receptor A, alpha 1 at ~51kD. Species Crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Tabuchi, Katsuhiko, et al. (2007). Science. 318: 71-6. 2. Miralles, et al. (1999). J. Comp. Neurol. 413: 535. 3. Li, M., A.L. De Blas. (1997). J. Biol. Chem. 272: 16564-16569. 4. Khan, Z., et al. (1994). J. Neurochem. 63: 371- 374. 5. Gutierrez, A., et al. (1994). J. Neurosci. 14: 7168-7179.