Technical Data
G1017-99
GAL3ST1 (3'-phosphoadenosine-5'-phosphosulfate:GalCer Sulfotransferase, 3'-phosphoadenylylsulfate:galactosylceramide 3'-sulfotransferase, Cerebroside Sulfotransferase, Galactosylceramide Sulfotransferase, GalCer Sulfotransferase, CST)
Description:
Sulfonation, an important step in the metabolism of many drugs, xenobiotics, hormones, and neurotransmitters, is catalyzed by sulfotransferases. GAL3ST1 is galactosylceramide sulfotransferase which catalyzes the conversion between 3'-phosphoadenylylsulfate + a galactosylceramide to adenosine 3',5'-bisphosphate + galactosylceramide sulfate. Activity of this sulfotransferase is enhanced in renal cell carcinoma.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:1000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:50-1:100
Flow Cytometry: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to aa95-124 from the center region of human GAL3ST1, conjugated to KLH.
Purity:
Purified by Protein A affinity chromatography
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human GAL3ST1. Species Crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Siegrist,H.P., et.al., Biochim. Biophys. Acta 489(1), 58-63 (1977). 2. Stein,C., et.al., J. Biol. Chem. 264(2), 1252-1259 (1989)