Technical Data
G1044-02
Galanthus nivalis (Snowdrop Bulb) (GNA)
Description:
Galanthus nivalis agglutinin (GNA) is a small molecular weight tetramer consisting of subunits of about 13,000D. This lectin contains little or no carbohydrate. Unlike most mannose-specific lectins, it is not a metalloprotein and does not require Ca++ or Mn++ for binding. Binding seems to be preferentially directed toward structures containing (a-1,3) mannose residues. In contrast to most mannose-binding lectins, GNA will not bind alpha linked glucose. Reports indicate that this lectin binds IgM, but not bind IgG immunoglobulin classes of rat and mouse. The only protein from human serum reported to bind to this lectin is a2-macroglobulin.

Applications:
Suitable for use in Immunohistochemistry and Immunocytochemistry. Also suitable for use in following lectin transport in neuronal tracing. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry: 1-10ug/ml
Immunocytochemistry: 1-10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceGoat
Concentration:
~2mg/ml (after reconstitution)
Immunogen:
Purified Galanthus nivalis lectin
Purity:
Purified by affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.8, 0.08% sodium azide and sucrose (added for stabilization). Reconstitute with 500ul sterile ddH2O. Do not vortex.
Specificity:
Recognizes Galanthus nivalis. Species crossreactivity: Narcissus pseudonarcissus (Daffodil) Lectin: 100%; Hippaestrum hybrid (Amaryllis) Lectin: 12%; Pisum sativum: 1%; Concanavalin A, lens culinaris: 1%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.