		Technical Data
G2005-02	Ganglioside GD2 (Disialoganglioside GD2)

Description:
GD2 has been identified as tumor-associated antigens expressed on the surface of neuroectoderm-derived tumors
(melanoma, neuroblastoma and glioma) and T-cell leukemia. Gangliosides (sialic acid-containing glycosphingolipids) are normal membrane constituents and highly expressed in the vertebral central nervous system.

Applications:
Suitable for use in Immunofluorescence, Flow Cytometry, Immunohistochemistry, Immunocytochemistry and Cytotoxicity. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1-2ug per 10e6 cells
Immunohistochemistry: Frozen tissue sections
Immunofluorescence: Cells in culture
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG2a	2Q549	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Human neuroblastoma cell line

Purity:

Purified by Protein A affinity chromotography.

Form

Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.

Specificity:

Recognizes human GD2 ganglioside.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Cheresh, D.A. et al. (1986) Cancer Res. 46: 5112–5118. 2. Mujoo, K. et al. (1987) Cancer Res. 47: 1098–1104. 3. Mujoo, K. et al. (1989) Cancer Res. 49: 2857–2861.