Technical Data
G2005-50A
Ganglioside GD3, O-acetyl
Description:
Gangliosides play vital roles in various cellular functions such as cell-cell adhesion, communication, and cell-substrate interactions. The divers sugar groups are generated by different types of O-substitutions, usually O-acetyl esters, of the parent molecules. 6A88 is raised against B49 cell line, and it specifically recognizes O-acetyl gangliosides. O-acetyl ganglioside is found in developing rat embryonic neuroectoderm and many human melanoma cells. In developing rat cerebellum, the O-acetyl ganglioside antigen was localized by immunofluorescence to germinal cells of the external granule layer. The 6A88 antigen is a marker for germinal cells, and the acquisition and subsequent loss of it is an aspect of the biochemical maturation of nervous and glial cells.

Applications:
Suitable for use in Immunohistochemistry and Immunofluorescence. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry (Frozen): 2-5ug/ml
Immunofluorescence: 2-5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgM6A88Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRatMouse
Concentration:
~0.5mg/ml
Immunogen:
B49 cells
Purity:
Purified by Protein A chromatography
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide
Specificity:
Recognizes O-acetyl gangliosides in rat brain tumor and most human malignant melanoma tumors. Does not crossreact with normal human skin melanocytes.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Blackburn CC, et al. JBC. 258: 1180-1188, 1983. 2. Cheresh DA, et al. JBC Vol. 259, No:12: 7453-7459, 1984. 3. Levine JM, et al. J Neurosci. 4(3): 820-831, 1984.