Technical Data
G2005-50A
Ganglioside GD3, O-acetyl
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Technical Data |
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G2005-50A |
Ganglioside GD3, O-acetyl |
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Description: Gangliosides play vital roles in various cellular functions such as cell-cell adhesion, communication, and cell-substrate interactions. The divers sugar groups are generated by different types of O-substitutions, usually O-acetyl esters, of the parent molecules. D1.1 is raised against B49 cell line, and it specifically recognizes O-acetyl gangliosides. O-acetyl ganglioside is found in developing rat embryonic neuroectoderm and many human melanoma cells. In developing rat cerebellum, the O-acetyl ganglioside antigen was localized by immunofluorescence to germinal cells of the external granule layer. The D1.1 antigen is a marker for germinal cells, and the acquisition and subsequent loss of it is an aspect of the biochemical maturation of nervous and glial cells. Applications: Suitable for use in Immunohistochemistry and Immunofluorescence. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (Frozen): 2-5ug/ml Immunofluorescence: 2-5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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