Technical Data
Ganglioside GD3
GD3 has been identified as tumor-associated antigens expressed on the surface of neuroectoderm-derived tumors (melanoma, neuroblastoma and glioma) and T-cell leukemia. Gangliosides (sialic acid-containing glycosphingolipids) are normal membrane constituents and highly expressed in the vertebral central nervous system.

Suitable for use in Flow Cytometry, Immunofluorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1-2ug/ml per 10e6 cells cells. Lysis of GD3 positive (melanoma) cells in presence of complement.
Immunohistochemistry: Frozen tissue sections
Immunofluorescence: Cells in culture
Optimal dilutions to be determined by the researcher.

Source: Ascites

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG32Q631Affinity Purified
100ug4C Do Not FreezeBlue IceHumanMouse
FM9 melanoma cells
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, pH 7.6, 0.09% sodium azide.
Recognizes human GD3 ganglioside1. Does not crossreact with other gangliosides purified from neuroblastoma or melanoma cells.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Cheresh, D.A. et al. (1984) Proc. Natl. Acad. Sci. U.S.A. 81: 5767-5771. 2. Cheresh, D.A. et al. (1985) Proc. Natl. Acad. Sci. U.S.A. 82: 5155-5159.