Technical Data
Ganglioside GM1 (Monosialoganglioside GM1) (Glycerol Free)
A member of the family of glycosphingolipids, monosialoganglioside acts as a receptor and antigen for cholera. GM1 ganglioside, one of the glycosphingolipids widely distributed in all tissues, occurs in highest concentrations in the central nervous system (CNS). It is primarily located in the outer surface of the mammalian cell's plasma membrane and in synaptic membranes of the CNS. GM1 ganglioside modulates a number of cell surface and receptor activities as well as neuronal differentiation and development, protein phosphorylation and synaptic function.

Suitable for use in ELISA and TLC immunoblotting with peroxidase reaction. Other applications have not been tested.

Recommended Dilutions:
ELISA: 1:1000. Optimal detection ca. 50ng of antigen.
TLC: 1:250
Note: Dilution in PBS, pH 7.4, is recommended.
Optimal dilutions to be determined by the researcher

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgG, IgMSerum
100ul-20°CBlue IceBovineRabbit
Not Determined
Purified monosialoganglioside GM1 and complete Freund’s adjuvant.
Supplied as a liquid. No preservative added. Glycerol free.
Recognizes bovine ganglioside GM1. Does not crossreact with other carbohydrate epitopes. Species Crossreactivity: Expresses some reactivity to asialo GM1 due to the natural background in rabbit. Expected to recognize GM1 ganglioside in most species with protein present.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Yoshino, H., et al., J. Neurochemistry 61: 658 (1993). 2. Kusunoki, S., et al., Neurology 37: 1795 (1987). 3. H. Yoshino, et al. Journal of Neurochemistry, Vol. 59(5):1684, 1992 4. A. Pestronk et al., Neurology, Vol. 49:1289, 1997