		Technical Data
G2006-89	Ganglioside GT1a (Trisialoganglioside GT1a, IV3NeuAcalpha2, II3NeuAca-Gg4Cer)

Description:
Applications:
Suitable for use in Immunohistochemistry , TLC-immunostaining and ELISA. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: Frozen sections
ELISA: 1:250-500.
TLC Immunostaining: 1:500 detects to about 0.05ug
Optimal dilutions determined by the researcher.

Localization:
Vertebral central nervous system

Source:
Ascites

Hybridoma:
PAI myeloma cells with spleen cells from C3H/HeN mice.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgM	3C95	Highly Purified

Size		Storage	Shipping	Source	Host
50ug		4°C (-20°C Glycerol)	Blue Ice		Mouse

Concentration:

~1mg/ml

Immunogen:

Purified ganglioside

Purity:

Purified by Cellulofine GCL-2000-sf gel filtration.

Form

Supplied as a liquid in PBS, 0.02% sodium azide before addition of glycerol to 40%.

Specificity:

Recognizes GT1a (IV3NeuAcalpha2, II3NeuAca-Gg4Cer). Crossreacts weakly with GT1b.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Kotani, M., et al., Biochem. Biophys. Acta, 1117: 97-103 (1992). 2. Kawashima, I., et al., J. Biochem., 114: 186-193 (1993). 3. Kotani, M., et al., Glycobiology, 4: 855-865 (1994). 4. Kotani, M., et al., Brain Res., 700: 40-58 (1995). 5. Kawashima, I., et al., Brain Res., 732: 75-86 (1996). 6. Tai, T., et al., Pure and Appl. Chem., 69: 1,903-1,910 (1997).