Technical Data
Media
GC Agar (Powder)
G2023
Microbiological Grade
Storage RT/4C    Shipping RT
Components are shown as g/Liter:
Peptic Digest of Animal Tissue7.5
Corn Starch1.0
Casein Digest Peptone7.5
Sodium Chloride5.0
Dipotassium Phosphate4.0
Monopotassium Phosphate1.0
Agar10.0
Total:36g/Liter
With addition of supplements and hemoglobin an enriched medium for growth of fastidious bacteria.

Description:
Various workers led to the development of an agar medium for the cultivation of Neisseria sp. Carpenter et al. increased the recovery time of Neisseria sp. by reducing the agar content. The enrichment of the medium with hemoglobin and yeast concentrate also greatly improved the growth of Neisseria sp. Heated hemoglobin releases hemin which is a growth factor for fastidious microorganisms such as Haemophilus sp. Cofactors, vitamins and nicotinamide adenine dinucleotide (required by Haemophilus sp.) are present in the chemical enrichment. Chocolate Agar is composed of GC Agar Base, hemoglobin and enrichment. Thayer-Martin Agar is Chocolate Agar with the addition of vancomycin to suppress Gram positive microorganisms, colistin to suppress Gram negative microorganisms and nystatin to inhibit yeast. Anisomycin is substituted for nystatin in Martin-Lewis agar.

Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.



Directions: 1. Dissolve 7.2grams per 100ml of distilled/ deionized (DDI) water, heating with stirring until completely solubilized.

2. Distribute and autoclave for at 121.0C for 15 minutes. Cool to room temperature. 3. Cool to 45-50C, mix 100ml of a 2% sterile hemoglobin solution, supplement and, if desired, selective antibiotics.Storage:Store powdered media at 4C. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be stored at 4C and used within a short period of time.

Microbiological Analysis: Haemophilus influenza ATCC 10211

Appearance: Light beige to beige, homogeneous, free flowing powder.

pH 3.6% (25C): 7.20.2


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.