		Technical Data
G2023-60	GCTM-5

Description:
The GCTM-5 monoclonal antibody reacts with a minority sub-population of cells in spontaneously differentiating cultures of pluripotent human embryonic stem cells and embryonal carcinoma. The epitope recognized by GCTM-5 is found on a 50kD protein present on the surface of these cells. Previous studies suggest that GCTM-5 may also prove to be a useful tool for defining cell lineage relationships between putative progenitor populations in embryonic liver and biliary epithelium during tissue repair (Pera M, et al, 2005).

Applications:
Suitable for use in Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Immunocytochemistry: 5-20ug/ml
Western Blot: 0.5-2.5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	9H238	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Membrane prepared from testicular seminoma.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

Specificity:

Recognizes human GCTM-5 antigen. The epitope recognized by GCTM-5 is found on a 50kD protein present on the surface of these cells.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Stamp L., Pera M, et al., (2005). Stem Cells. 23(1):103-112. 2. Tosh D, et al., (2005). Hepatology. 42(1):S75-S84. 3. Costa M, et al., (2005). Nat Methods. Apr 2, 2(4):259-260. 4. Laslett A, et al., (2007). BMC Dev Biol. Mar 2, 7(1):12. 5. Andrews P, et al., (2007). Nat Biotechnol. Jun 17, 25(7):803-816.