Technical Data
G2032-25H
GFAP (Glial Fibrillary Acidic Protein, Astrocyte)
Description:
GFAP is a 50kD intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas. At least 3 isoforms are produced from alternate splicing. These isoforms differ in the C-terminal region which is encoded by alternative exons. This antibody detects all three isoforms.

Applications:
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-1ug/ml
Immunohistochemistry (Formali fixed paraffin, formalin/acetone fized tissues): 0.5-1ug/ml
Optimal dilution determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG9G362Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IcePorcineMouse
Concentration:
~0.1mg/ml
Immunogen:
GFAP from porcine spinal cord.
Purity:
Purified by affinity chromatography.
Form
Supplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.2% sodium azide. Reconstitute with 1ml sterile PBS.
Specificity:
Recognizes porcine GFAP at ~50kD. Species Crossreactivity: human, mouse, rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.