Technical Data
G2032-25X
GFAP (Glial Fibrillary Acidic Protein) (BSA & Azide Free)
Description:
Glial fibrillary acidic protein is specific to astrocytes and ependylmal cells of the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the central nervous system.

Applications:
Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Immunoprecipitation (Native and denatured): 2ug/mg protein lysate (Use Protein G).
Western Blot: 1-2ug/ml for 2 hrs at RT
Immunohistochemistry (formalin/paraffin): 1-2ug/ml for 30 min at RT. No special pretreatment is required for staining of formalin-fixed tissues.
Optimal dilutions to be determined by the researcher.

Source: Ascites

Positive Control: Astrocytoma, IMR5 and brain cells.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG10.N.308Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IcePorcineMouse
Concentration:
~1mg/ml
Immunogen:
Porcine spinal cord. Cellular Localization: Cytoplasmic.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and sodium azide. See G2032-25.
Specificity:
Recognizes porcine glial fibrillary acidic protein (GFAP) at 51-52kD. Stains astrocytes, glial cells, ependymal cells and their corresponding tumors. Many types of neural tumors such as neuroblastomas Schwannamoas, as well as extra-CNS tumors are not labeled. Species Crossreactivity: human, rat and chicken.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Debus, E., et al., Differentiation 25(2): 193-203 (1983). 2. Yachnis, A.T., et al., J. Comp. Neurol. 334: 356-369 (1993). 3. Trivino, A., et al., Vision Research 32: 1601-1607 (1992