Technical Data
G2032-27H
GFAP (Glial Fibrillary Acidic Protein)
Description:
Human GFAP is a 49.7kD protein (322 amino acids) expressed in astrocytes. GFAP is an intermediate filament protein and acts as an intracellular structural component of the cytoskeleton. During embryonic and fetal life, GFAP is also expressed by radial glial cells of the CNS. Various mutations of the GFAP genes in humans produce Alexander’s disease, one of the leukodystrophies.

Applications:
Suitable for use in Immunocytochemistry, Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunocytochemistry: 1:100-1:200
Immunofluorescence: 1:1000
Western Blot: 1:500
Immunohistochemistry: 1:100-1:200
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1906: IgY, Chicken Pab Rb x Ch
I1906-10: IgY, Chicken (HRP) Rb x Ch
I1906-12: IgY, Chicken (FITC) Rb x Ch
I1906-14: IgY, Chicken (AP) Rb x Ch

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgYAffinity Purified
SizeStorageShippingSourceHost
100ul-20°CBlue IceMouseChicken
Concentration:
~1mg/ml
Immunogen:
Recombinant protein corresponding to mouse GFAP.
Purity:
Purified by affinity chromatography
Form
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity:
Recognizes mouse GFAP. Species Crossreactivity: human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. David H. McDougal,Gerlinda E. Hermann, and Richard C. Rogers. Vagal Afferent Stimulation Activates Astrocytes in the Nucleus of the Solitary Tract Via AMPA Receptors: Evidence of an Atypical Neural-Glial Interaction in the Brainstem. The Journal of Neuroscience, 28 September 2011, 31(39): 14037-14045; doi: 10.1523/JNEUROSCI.2855-11.2011. 2. Sue H. Huh, Young C. Chung, Ying Piao, Min Y. Jin, Hyo J. Son, Nam S. Yoon, Joo Y. Hong, Youngmi K., Pak, Yoon S. Kim, Jong K. Hong, Onyou Hwang#and Byung K. Jin. Ethyl Pyruvate Rescues Nigrostriatal Dopaminergic Neurons by Regulating Glial Activation in a Mouse Model of Parkinson’s Disease. Published online before print June 17, 2011, doi: 10.4049/The Journal of Immunology.