Technical Data
G2033-53D
GFRA3, ID (GDNF Family Receptor alpha-3, GDNF Receptor alpha-3, GDNFR-alpha-3, GFR-alpha-3, UNQ339/PRO538/PRO3664)
Description:
Members of the glial cell line-derived neurotrophic factor (GDNF) family including GDNF and neurturin (NTN) play essential roles in the control of vertebrate neuron survival and differentiation. A new member of the GDNF family was recently identified and designated persephin. Physiological responses to these neurotrophic factors require two receptor subunits, the novel glycosylphosphadidylinositol linked protein GFRa and Ret receptor tyrosine kinase GFRb. Following the findings of GFRa-1 and -2, a novel receptor in GFRa family was identified very recently from human and mouse and designated GFRa-3. GFRa-3 binds persephin, thus, persephin, GFRa-3, and Ret PTK form a complex to transduce persephin signal and to mediate persephin function.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
3T3 cell lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceMouseRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to 14aa from near the internal sequence of mouse GFRa-3.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes mouse GFRa-3. Species Crossreactivity: human and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Milbrandt, J. et al. Neuron 20, 245 (1998). 2. Nomeclature Committee. et al. Neuron 19, 485 (1997). 3. Jing, S. et al. J. Biol. Chem. 272, 33111 (1997). 4. Baloh, RH. et al. Proc. Natl. Acad. Sci. USA 95, 5801 (1998). 5. Worby, CA. et al. J. Biol. Chem. 273, 3502 (1998). 6 Naveilhan, P. et al. Proc. Natl. Acad. Sci. USA 95, 1295 (1998). 7. Nomoto, S. et al. Biochem. Biophys. Res. Commun. 244, 849 (1998).