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Technical Data |
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G2036-05 |
Glial Derived Neurotrophic Factor (GDNF) |
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Description: Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: direct ELISA: 0.5ug/ml using 100ul/well allows the detection of 0.2–0.4 ng/well of recombinant hGDNF. Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGDNF is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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