Technical Data
Glial Derived Neurotrophic Factor, Recombinant, Human (GDNF)
Molecular Biology Storage: -20CShipping: Blue Ice
Human GDNF (Glial-Derived Neurotrophic Factor) is a 30.1kD protein that specifically promotes dopamine uptake and survival of midbrain neurons. GDNF is a disulphide-linked homodimer, formed by two identical 134aa subunits.

Recombinant corresponding to human GDNF expressed in E. coli.

Biological Activity:
The ED50 as determined by the dose-dependent dopamine uptake by rat ventral mesencephalic cultures was found to be in the range of 4-8ng/ml.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: E.coli
Purity: ~98% (SDS-PAGE and HPLC). Endotoxin: <0.1EU/ug
Concentration: Not determined
Form: Supplied as a lyophilized powder from 10mM sodium citrate, 150mM sodium chloride, pH 4. Reconstitute in sterile ddH2O to 0.1-1.0mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.