		Technical Data
G2040-01	Glucagon

Description:
Glucagon is produced by the alpha-cells of the pancreatic islets. It occurs also in endocrine cells of the gut and in neurons in the brain.

Applications:
Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (PAP): 1:1000-1:2000; requires overnight incubation at 4ºC.
Note: Suitable for frozen and paraffin-embedded sections. No proteolytic treatment is required.
Optimal dilutions to be determined by the researcher.

Positive Control:
Bouin-fixed paraffin sections of pancreas

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
50ul		-20°C	Blue Ice	Porcine	Guinea pig

Concentration:

Not Determined

Immunogen:

Highly purified porcine pancreatic glucagon. Cellular Localization: Cytoplasmic

Purity:

Serum

Form

Supplied as a lyophilized powder. Reconstitute in 50-100ul sterile ddH2O. Dilute further in 0.1M PBS, 1% BSA, 0.1% sodium azide.

Specificity:

Recognizes porcine Glucagon. Can be used for the detection of pancreatic endocrine tumors. Absorption with 10-100ul glucagon per ml diluted antiserum abolishes staining. Species Crossreactivity: human and rat

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Myrsen-Axcrona, U., et al., Regulary Peptides 68: 165-175 (1997).