Technical Data
G3056-02
GPI (Neuroleukin, sperm antigen-36, phosphosaccharomutase, phosphohexose isomerase, phosphohexomutase, phosphoglucose isomerase, oxoisomerase, neuroleukin, hexosephosphate isomerase;, hexose monophosphate isomerase, glucose-6-phosphate isomerase, autocrin
Description:
This gene belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.

Applications:
Suitable for use in ELISA and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Peptide ELISA Titer: 1:128,000
Western Blot: 0.01-0.03ug/ml ~ 60kD band observed in Human Heart lysates (calculated MW of 63.1kD according to NP_000166.2).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-YREHRSELNLRR, from the internal region (near N Terminus) of human GPI (NP_000166.2).
Purity:
Purified by peptide affinity chromatography.
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes human GPI. Species sequence homology: porcine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Iwanami K, Matsumoto I, Tanaka Y, Inoue A, Goto D, Ito S, Tsutsumi A, Sumida T, Arthritogenic T cell epitope in glucose-6-phosphate isomerase-induced arthritis. Arthritis research & therapy 2008 10 (6): R130.