Technical Data
G3057-12B
Glucose Regulated Protein 75 (Grp75, HSPA9, heat shock 70kDa protein 9 (mortalin), CSA, HSPA9B, MGC4500, Mortalin, MOT, MOT2, mot-2, mthsp75, MTHSP75, PBP74, Peptide-binding protein 74, Stress-70 protein, mitochondrial)
Description:
Grp75, also known as mortalin, is a member of Hsp70 family of chaperone proteins that is not heat inducible (1, 2). Grp75 is actually induced under conditions of low glucose and other nutritional and environmental stresses. Grp75 resides primarily in the mitochondrial matrix,
where it collaborates with Hsp60 in the re-folding of proteins translocated into this organelle (3, 4). Related forms may also be found in the cytosol or on the surface of the extracellular membrane. Other Grp75 functions include its ability to inactivate the tumor suppressor p53 (5). Studies have found that Grp75 is over-expressed in many tumor tissues and immortalized human cell lines, suggesting its role in the tumor formation (6). Grp75 is also implicated in cell
aging, as its overexpression appears to prolong the life span of human fibroblasts (7). And finally, like its E.coli homolog DnaK (8), GRP75 possesses a cation-dependent ATPase activity considered central to its function as a chaperone (9, 10).

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG110F705Affinity Purified
SizeStorageShippingSourceHost
25ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to human Grp75.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
Specificity:
Recognizes Grp75. SpeciesCcross-Reactivity: rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kaul S.C., et al. (1993) Biochem Biophys Res Commun. 193: 348-355. 2. Wadhwa R., et al. (1993) J Biol Chem 268: 6615-6621. 3. Schneider H.C., et al. (1994) Nature 371: 768-774. 4. Manning-Krieg U.C., et al. (1991) EMBO J. 10: 3273-3280. 5. Wadhwa R., et al. (1998) J Biol Chem. 273: 29586-91.
6. Wadhwa R., et al. (2006) Int J Cancer 118: 2973-2980. 7. Kaul S.C., et al. (2003) Exp Cell Res. 286: 96-110. 8. Liberek K., et al. (1991) J Biol Chem. 266: 14491-14496. 9. Mizzen L.A., et al. (1991) Cell Regulation. 2: 165-179. 10. Leustek U.K., et al. (1989) PNAS USA. 86: 7805-7808.